Xenbase Image ID: 171549

Figure 4. Regulation of the cell cycle by B55α and B55β. (A) Metaphase-arrested Xenopus egg extracts were supplemented with recombinant B55α or control buffer for 10 min. These extracts were then released into interphase by the addition of Calcium at time 0. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Phosphorylation (band-shift) of Cdc27 and Cdc25, and the global phosphorylation of Cdk substrates are markers of mitosis. (B) Interphase Xenopus egg extracts were supplemented with recombinant B55α or control buffer. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Mitosis is indicated by Cdc27 phosphorylation. (C) MBP-B55α and B55β were purified and examined by Coomassie staining. (D) Interphase Xenopus egg extracts were supplemented with recombinant B55α, B55β, or control buffer. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Mitosis is indicated by Cdc27 phosphorylation. Image published in: Wang F et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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