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Figure 5. RPA and B55α association. (A) RPA2 IP was performed in HeLa cell lysates as described in Material and Methods. The input at 10%, control IP (with blank beads), and RPA2 IP were analyzed by immunoblotting for B55α and RPA2. (B) B55α IP was performed in HeLa cell lysates. The input at 10%, control IP (with blank beads), and B55α IP were analyzed by immunoblotting for B55α and RPA2. (C) HA-B55α was expressed in HeLa cells, which were treated with or without HU (1 mM, 24 h). HA IP was performed in the cell lysates. The input at 10%, control IP (with blank beads), and B55α IP were analyzed by immunoblotting for B55α and RPA2. (D) RPA2 IP was performed in the lysates of HeLa cells that were pre-treated with HU (1 mM, 24 h) or mock-treated. The input at 10%, control IP (with blank beads), and B55α IP were analyzed by immunoblotting for B55α and RPA2. (E) The level of B55α in RPA2 IP was examined by immunoblotting and quantified using ImageJ. The mean values and standard deviations were calculated from three independent experiments. Statistical significance was analyzed using an unpaired 2-tailed Student’s t-test. A p-value < 0.05 was considered statistically significant (*). (F) The expression level of B55α was examined by immunoblotting and quantified using ImageJ. The mean values and standard deviations were calculated from three independent experiments. Statistical significance was analyzed using an unpaired 2-tailed Student’s t-test. A p-value < 0.05 was considered statistically significant (*). Image published in: Wang F et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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