XB-IMG-171617
Xenbase Image ID: 171617
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Figure S4 (related to Figure 5): Comparison of number of detections per cluster for both
Nup93 and Nup188 in 2D-FPALM and fluorescence intensity in STED.
(A) Two-color 2D-FPALM image of cell labeled with anti-AcTub (green) and anti-Nup93 (red).
Three boxed ROIs from the nuclear surface, the cilium base, and the cellular background are
magnified in B-D.
(B-D) Magnification of the three ROIs shown in panel A showing number of localizations for a
selected number of clusters (white boxes) in all three ROIs.
(E) STED images of immunolabeled anti-Nup188 clusters at the cilium base. ROIs are
selected from both the cilium base and the cellular background to show the increased
fluorescence intensity (in arbitrary units) of clusters at the basal body (far right panels, white
boxes). Diffraction limited confocal images are also shown in middle panels. Image published in: Del Viso F et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |