Xenbase Image ID: 171627

Figure 6. Nup188 Forms Two Barrel-like Structures that Encase the Centrioles (A) Diffraction-limited (inset) and 3D W-4PiSMSN super-resolution image of anti-Nup188 signal at the cilium base. Heatmap coloration reflects depth in z as depicted by scale. Individual structures are circled. See also Movie S2. (B) Nearest-neighbor distances from all localiza- tion clusters in all structures imaged were calcu- lated and plotted. (C–E) Rotated views of image in (A) where two cylinders (blue and red) have been fitted into both structures denoted by ‘‘1’’ and ‘‘2.’’ See also Movie S2. (F–I) Planar sections of each structure cut across the cylinder axis (central point). Dotted lines represent cylinders. (J and K) Distribution of the distances of individual localizations from W-4PiSMSN data inside (nega- tive values) and outside (positive values) the modeled cylinder surface of structure 1 (J) and structure 2 (K). (L) QR code to load Nup188 W-4PiSMSN structure into the 3D visualization application Augment. See Experimental Procedures for instructions. See also Figures S5 and S6. Image published in: Del Viso F et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page