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Figure 2—figure supplement 1. Validation of changes in synthesis of eIF3A, FUS, and RPS17 in response to 4 hr of visual experience.(A) Protocol to prepare AHA-labeled samples for western blot. (B, C) Scatter plots of western blot data of newly synthesized (B) or total (C) CPPs. Data are normalized intensity ratios of VE samples compared to the paired controls. The intensities of individual bands for total proteins were normalized to β-tubulin, while the intensities of bands for NSPs were normalized to biotinylated BSA, which served as an internal standard, and β-tubulin. Western blots of CPPs (red) corroborated the proteomic results. *p<0.05, **p<0.01, two-tailed Student’s t test for comparisons of total proteins and Mann-Whitney test for comparisons of NSPs. n ≥ 4 independent experiments for each CPP. The black bars represent the mean. Image published in: Liu HH et al. (2018) © 2018, Liu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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