XB-IMG-171670
Xenbase Image ID: 171670
Figure 4—figure supplement 1. Newly synthesized FUS is required for visual experience-dependent structural plasticity.(A–D) To test the effect of overexpression of CPPs on structural plasticity in response to VE, tecta were electroporated with GFP-expression plasmid 2 days before imaging. GFP-expressing tectal neurons were imaged in vivo before and after 4 hr in dark followed by 4 hr of VE. Dendritic arbors of individual neurons were reconstructed in 3D and total dendritic branch length (TDBL) was compared across imaging time-points for individual neurons. (A–D) Plots of VE-dependent changes in tectal neuron growth rate over 4 hr in dark and VE in tecta electroporated with GFP-expression plasmid (A), GFP and eIF3A-expression plasmid (B), GFP and FUS-expression plasmid (C), or GFP and RPS17-expression plasmid (D). Gray lines connect data points for individual neurons and black lines are the average changes in growth rate in dark and VE. Neurons treated with control GFP-expression plasmid increase growth rate with VE compared to dark. Overexpression of RPS17 blocked the VE-dependent increase in dendritic arbor growth. VE-dependent structural plasticity was unaffected by eIF3A or FUS overexpression. Control: n = 8 cells; eIF3A OE: n = 6 cells; FUS OE: n = 11 cells; RPS17 OE: n = 17 cells. *p<0.05, **p<0.01, Two-tailed paired Student’s t test were used to compare between two matched pairs. Image published in: Liu HH et al. (2018) © 2018, Liu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |