XB-IMG-171672
Xenbase Image ID: 171672
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Figure 6—figure supplement 1. eIF3A and RPS17 regulate protein synthesis in the tadpole tectum.(A) Schematic of tadpole optic tectum showing the location of neuronal cell body layer (green) where tectal neuronal somata (black) extend processes into the neuropil (gray). MOs were electroporated into the tectum, and two day later AHA was injected into the midbrain ventricle. Tadpole brains were dissected 1 hr later and processed for click chemistry, which tags AHA with a fluorophore for visualization of AHA-labeled proteins. (B) Images of fluorescent AHA labeling in z-projections through optic tectum (top) or single confocal optical sections from comparable depths in optic tectum (bottom) from animals electroporated with control MO, eIF3A MO, RPS17 MO, or both eIF3A and RPS17 MO. In vivo AHA labeling detected 1 hr after ventricular AHA injection is increased by knockdown of eIF3A in both the neuronal cell body layer and neuropil. Knockdown both eIF3A and RPS17 only increase AHA labeling in the neuropil. (C) Normalized AHA labeling in the neuronal cell body layer and neuropil in animals treated with control MO, eIF3A MO, RPS17 MO, or both eIF3A and RPS17 MO. Control MO: 1 ± 0.04 in neuronal cell body layer and 1 ± 0.04 in neuropil, n = 14 brains; eIF3A MO: 1.91 ± 0.15 in neuronal cell body layer and 1.81 ± 0.3 in neuropil, n = 7 brains; RPS17 MO: 1 ± 0.07 in neuronal cell body layer and 1.21 ± 0.09 in neuropil, n = 12 brains; eIF3A plus RPS17 MO: 1.13 ± 0.05 in neuronal cell body layer and 1.36 ± 0.09 in neuropil, n = 19 brains. *=p < 0.05, **=p < 0.01, Steel-Dwass test was used for nonparametric multiple comparisons between all pairs. Error bars represent ±SEM. Scale bar = 100 μm. Image published in: Liu HH et al. (2018) © 2018, Liu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |