Xenbase Image ID: 171714

Fig. 2. Chromatin binding of HELLS–CDCA7e is coregulated by the CPC and nucleosomes. (A) Unsupervised clustering of chromatin-associated proteins. A heatmap of each protein’s normalized profile is displayed, with proteins enriched on a given chromatin sample colored yellow. Examples of known stoichiometric chromatin-associated complexes cluster together and are indicated. Fine-grained protein complex clusters (Left) and coarse-grained cell-cycle clusters (Right) are labeled. (B) LC-MS/MS analysis HELLS and CDCA7e copurified with unmodified or H3K9me3 nucleosome beads recovered from M phase or interphase mock or CPC-depleted extracts. (C) Western blot analyses of HELLS and CDCA7e copurified with nucleosome beads recovered from M phase or interphase mock or CPC-depleted extracts. Representative of n = 2 independent experiments. (D) Western blot analyses of proteins copurified with nucleosome beads recovered from M phase mock or CPC-depleted extracts. Nucleosomes containing wild-type H3 (SS), H3S10AS28A (AA), or H3S10DS28D (DD) were used. (E) Western blot analyses of proteins copurified with nucleosome or DNA beads recovered from M phase or interphase H3–H4-depleted extracts. (F) Abundance of HELLS and CDCA7e on nucleosome or DNA beads recovered from M phase H3–H4-depleted extracts quantified by LC-MS/MS. Data from ref. 28 (originally missing CDCA7e) was reanalyzed using an mRNA reference database containing CDCA7e (34). Image published in: Jenness C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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