Xenbase Image ID: 171715

Fig. 3. CDCA7e directly recruits HELLS to chromatin. (A and B) Western blot analysis of CDCA7e (A) and HELLS (B) immunoprecipitation from M phase Xenopus extracts. Preimmune rabbit IgG was used to control for nonspecific binding. Representative of n = 3 independent experiments. (C) Coomassie-stained gel of purified HELLS–CBP coimmunoprecipitation with MBP–CDCA7. Purified HELLS–CBP was incubated with MBP–CDCA7e or MBP alone and immunoisolation was performed using beads coupled with anti-MBP antibodies or control IgG. Representative of n = 2 independent experiments. (D) Western blot analyses of proteins copurified with nucleosome beads recovered from interphase extracts mock depleted or depleted of HELLS or CDCA7e. Representative of n = 4 independent experiments. (E) Abundance of HELLS and CDCA7e on nucleosome beads recovered from interphase or M phase extracts mock depleted or depleted of HELLS or CDCA7e, quantified by LC-MS/MS. (F) Coomassie-stained gel of a pulldown of nucleosome or DNA beads incubated with MBP–CDCA7e. Uncoupled beads were used to control for nonspecific binding. Representative of n = 2 independent experiments. Image published in: Jenness C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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