Xenbase Image ID: 171721

Fig. S1. [panels G-K] Proteomic profiling of chromatin regulation by the cell cycle, CPC, and H3K9me3 to identify how protein complexes are regulated. (A–E) Abundance of HP1-gamma-SUV39H1 (A), ATRX–DAXX (B), SSRP1–SPT16 (C), FANCI–D2 (D), and Astrin–SKAP (E) identified by LC-MS/MS on the indicated chromatin beads. Abundance (arbitrary units) was calculated by integrating LC-MS signals for each peptide, as described in Methods. (F) Abundance of the indicated protein complexes on M phase and interphase chromatin identified by LC-MS/MS. (G–K) Dendrogram of the mitosis-specific branch (F), general branch (H and J), M phase enriched branch (I), or HELLS–CDCA7 branch (K) of hierarchical clustering shown in Fig. 2A. A heatmap of each protein’s normalized profile is displayed, with proteins enriched on a given chromatin sample colored yellow. Note the condensin subunits cluster within the larger mitosis-specific cluster. Image published in: Jenness C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page