Xenbase Image ID: 171722

Fig. S2. HELLS and CDCA7e are stoichiometrically equivalent on chromatin beads. (A) Quantification of Western blot bands of CDCA7e and HELLS in Xenopus egg extract. The amount loaded of each protein was calculated from their concentration in extract (34). (B) Western blot quantification of the amount of HELLS and CDCA7e on nucleosome beads recovered from interphase extract. Each connected pair of dots is an independent incubation of chromatin beads in extract. Wilcoxon matched pairs test was used to determine significance. (C) Western blots used to quantify A and B Image published in: Jenness C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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