Xenbase Image ID: 171723

Fig. S3. [panel A B C] Characterization of HELLS and CDCA7 interaction and chromatin binding. (A) Coomassie-stained gel of purified MBP–CDCA7e and HELLS–CBP used in this study. (B) Coomassie-stained gel of a pulldown of 19 × 601 DNA beads incubated with MBP, MBP–CDCA7e, or HELLS–CBP. Uncoupled beads were used to control for nonspecific binding. (C) Western blot analysis of HELLS coimmunoprecipitation with CDCA7 paralogs. MYC–CDCA7 paralogs were expressed from mRNA in interphase Xenopus egg extract. Immunoprecipitation was performed using beads coupled with anti-HELLS antibodies or control IgG. Representative of n = 2 independent experiments. (D) Coomassie-stained gel of a pulldown of mononucleosomes with 5 bp 5′ linker or the naked DNA equivalent incubated with MBP–CDCA7e. Uncoupled beads were used to control for nonspecific binding. Image published in: Jenness C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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