Xenbase Image ID: 171774

Figure 1. TALEN-Induced Deletions Nullify Brachyury Function (A) TALEN-induced 2- and 7-bp deletions in exon 1 of t (e1.2D) and exon 3 of t2 (e3.7D), and predicted frameshift translations generating truncated proteins of 59 and 170 amino acids (aa). These mutations were selected to generate a double heterozygous X. tropicalis line for the Brachyury paralogs t and t2 (te1.2D/+t2e3.7D/+). (B) t and t2 transcript levels in hetero- and homozygous embryos as measured by qRT-PCR at early neurula stage (n = 3, mean ± SD). Two-tailed t test: ∗p ≤ 0.05. (C) Multi-probe WMISH for various mesoderm cell lineage and derivative markers (actc1; cardiac and skeletal muscle; cav1, notochord; hoxd8, pronephros; myh6, heart; tal1, ventral blood island; tbx6, paraxial mesoderm) in wild-type and Brachyury (t/t2) null (KO) embryos, as well as embryos injected with four MOs targeting t and t2 (t/t2 MO mix) at mid-tailbud stage. Scale bar, 0.5 mm. Image published in: Gentsch GE et al. (2018) © 2018 The Francis Crick Institute. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue actc1.L actc, actna1, cardiac actin, cardiac alpha actin, muscle actin X. laevis Throughout NF stage 28 heart somite cav1.L bscl3, Cav-1, cav1-a, cav1-b, caveolin-1, cgl3, vip21 X. laevis Throughout NF stage 28 notochord hoxd8.L hox5.4, hoxd-8 X. laevis Throughout NF stage 28 pronephric kidney pronephric duct tal1.L bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 28 ventral blood island tbx6.L XTbx6 X. laevis Throughout NF stage 28 paraxial mesoderm myh6.L aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 28 heart Image source: Published Larger Image Printer Friendly View

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