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Figure 4. Intensity of Innate Immune Response Depends on GC Content of MO (A) Assigning the induction of immune response-related genes tp53, tp53inp1, and c3ar1 to single splice- or translation-blocking MOs (MOsplice and MOtransl) of the t/t2 MO mix injected at 8 ng per embryo as well as to the cell lineage tracer sulforhodamine (coupled to dextran). The transcript fold change was determined at late tailbud stage by qRT-PCR (n = 4, mean ± SD). Two-tailed t test: ∗p ≤ 0.1. (B and C) c3ar1 induction was increasing with the GC content of the injected MO as determined by qRT-PCR (n = 4, mean ± SD) and WMISH. Scale bar, 0.5 mm. Image published in: Gentsch GE et al. (2018) © 2018 The Francis Crick Institute. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue c3ar1.L C3aR X. laevis Throughout NF stage 33 and 34   Image source: Published Larger Image Printer Friendly View

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