XB-IMG-171781
Xenbase Image ID: 171781
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Figure S2. Brachyury KO and KD Embryos Are Morphologically Very Similar,
Related to Figure 2
(A) Gross morphological comparison between different conditions of the KD and KO experiment at the indicated tailbud stages. Uninjected (uni) and control MO (cMO)-injected embryos were controls for the t/t2 morphants (t/t2 MO). Wild-type (wt)embryos were siblings of the mutant embryos (hetero- and homozygous for the mutant and t2 allele, t/t2 het and t/t2 KO). Scale bar, 0.5 mm. (B) Comparison of low fragment count gene transcripts to estimate minimal fragment count required for calling reliable fold changes. Genes that averaged <7 fragments between cMO-injected and uninjected and heterozygous and wild-type embryos over tailbud stage 26 and 34 were excluded due to a higher degree of spurious fold changes. Image published in: Gentsch GE et al. (2018) © 2018 The Francis Crick Institute. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |