Xenbase Image ID: 171782

Figure S3. Increased Transcription of tp53 Depending on GC Content of MO Does Not Cause More Apoptosis, Related to Figure 3 and 4 (A) TUNEL assay on morphants and sibling embryos from double heterozygous t -/+t2+/- parents. DNase-treated wild-type embryos were used as positive controls. (B) Single WMISH for tp53 and multi-probe WMISH for various mesoderm cell lineage and derivative markers (cav1, notochord; hoxd8, pronephros; myh6, heart; tal1, ventral blood island; tbx6, paraxial mesoderm) of late tailbud embryos injected with single MOs or tracer sulforhodamine-dextran. Scale bar, 0.5 mm Image published in: Gentsch GE et al. (2018) © 2018 The Francis Crick Institute. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tp53.L p53, Trp248, tumor suppressor p53, Xp53, Xrel3 X. laevis Throughout NF stage 33 and 34 pharyngeal region hindbrain hoxd8.L hox5.4, hoxd-8 X. laevis Throughout NF stage 33 and 34 pronephric kidney pronephric tubule myh6.L aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 33 and 34 heart tal1.L bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 33 and 34 ventral blood island tbx6.L XTbx6 X. laevis Throughout NF stage 33 and 34 tail bud Image source: Published Larger Image Printer Friendly View

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