XB-IMG-171783
Xenbase Image ID: 171783
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Figure S4. Specific MOs of the t/t2 MO Cocktail Cause Off-Target Splicing
Defects, Related to Figure 5
(A,C) Superimposed Sashimi plot of abi1 and bloc1s4 transcripts whose splicing was
perturbed by the injected t/t2 MO mix. Canonical and alternative splicing are shown
with solid and dashed lines, respectively. Blocked splice sites containing matches of
≥8 consecutive bases with a specific MO are shown as alignments. Canonical WatsonCrick
and non-canonical wobble base pairing are marked as vertical bar and colon,
respectively. (B,D) RT-qPCR (n = 4, mean ± SD) confirmed that specific MOs of the
t/t2 MO mix were responsible for mis-splicing. The fold change (log2 scale) of transcript
levels and alternative splicing between exon 7 and 11 (abi1) and exon 4 and 6
(bloc1s4) are shown as filled and solid bars, respectively. Two-tailed t-test: *, p ≤0.1;
**, p ≤0.01. See Figure 3D and Key Resources Table for the design of RT-qPCR
primers. Image published in: Gentsch GE et al. (2018) © 2018 The Francis Crick Institute. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |