XB-IMG-171800
Xenbase Image ID: 171800
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FIGURE 4. (A) The nucleotide and deduced amino acid sequences are shown for segments of two isoforms of Xenopus Msi-2 detected by RT-PCR. These isoforms were detected using one set of forward and reverse primers. The arrows under the nucleotide sequence show the position of forward (5′-CTCTATGGAACTGCCAGCCAGGA-3′) and reverse (5′-GATGCCCAAGCAAGACTGGGAG-3′) primers sequences. The longer isoform (A1) yielded a 203 bp band, while the shorter isoform band (A2) had 145 bp. The splicing insert (underlined DNA sequence) in the long isoform (A1) occurred between the greater-than (>>>>>) and the less-than signs (<<<<<). In the shorter isoform (A2), the region underlined in A1 was spliced out and the regions designated between the greater-than the less-than signs, respectively, were directly joined. The bracketed numbers above some amino acids indicate the differing region, and correspond to schematic (A). The asterisks in A1 and A2 show the terminal codons. (B) The schematic for the deduced amino acid sequence region illustrates 100% homology from amino acid 1 to 393, between the longer and shorter band isoforms, with the alternatively spliced region from 394 to 406. The full length amino acid sequence of both isoforms has the same 406 amino acid length. RRM1 and RRM2 indicate, respectively, RNA-recognition motif-1 and -2. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |