Xenbase Image ID: 171801

FIGURE 5. Xenopus Msi-1 and Msi-2 semi-quantitative RT-PCR gels show strong expression of Msi-1 in intact, regeneration-competent NF 50 spinal cord (gel column 1), and 5-day regenerating NF 50 spinal cord (Reg. 5D NF 50 spinal cord, gel column 3). Both Msi-1 and Msi-2 were reduced from NF 50 levels in intact NF 62 (non-regeneration-competent) and adult spinal cord (compare gel columns 1, 2). Msi-2 specific primers showed bands representing the two isoforms shown in the Figure 4 alignments (arrowheads). The larger isoform was universally present. The smaller isoform (lower arrowhead) was present only in NF 62 and adult frog (compare gel columns 2, 4). GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as the housekeeping control gene. The No-RT control (–RT) was performed without reverse transcriptase using the GAPDH primers. Each gene is shown in a separate row. All bands in each row are from one original gel. Negative was shown as for image clarity. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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