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FIGURE 6. Axolotl Msi semi-quantitative RT-PCR gel. Msi-1 was strongly expressed in the pre-hatching/late tailbud stage embryo (BD 42–44; column 1), juvenile brain and spinal cord (gel columns 2, 3), but was absent in intact adult spinal cord (column 4). At 3 weeks post-transection in the adult (3 weeks regenerating adult cord) Msi-1was up-regulated (column 5). Msi-2 expression was stronger in the juvenile brain and spinal cord (columns 2, 3) than in the BD 42–44 embryo (column 1). Msi-2 was still expressed in intact adult spinal cord (column 4), and not up-regulated in regenerating adult cord (column 5). GAPDH was used as the internal housekeeping gene loading control, and (–RT) was performed without reverse transcriptase using the GAPDH primers. Each gene is shown in a separate row. All bands in each row are from one original gel (see Supplementary Figure 2) with limb regeneration sample bands excised here. Negative was shown as for image clarity. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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