Xenbase Image ID: 171803

FIGURE 7. mRNA tissue distribution of Xenopus Msi-1 in developing and regenerating spinal cord using section in situ hybridization (blue reaction product). (A) Cross section of intact NF 50 spinal cord. Msi-1 was strongly expressed in the ependymal layer. (B) Shh mRNA localization in intact NF 50 spinal cord in the section adjacent to the one shown in “A.” Shh is expressed in the floorplate (arrow). (C) NF 62 spinal cord cross section showed Msi-1 was still expressed in the ependymal layer, but mostly ventrally located and weaker. (D) NF 62+ Shh mRNA is still expressed in the ventral floorplate, overlapping the Msi-1 expression (arrow). (E) Parasagittal section of NF 52 spinal cord 1-day (1-D) post-transection showed high level of Msi-1 expression throughout the cord, lesion site and caudal stump. (F) Cross section of NF 52 regenerating cord 5-days (5-D) post-transection showed a broad ventral arch of Msi-1 expression in the ependymal outgrowth. The dorsal portion of the notochord is visible below the outgrowth (noto). (G) Cross section of NF 52 regenerating cord 5-days (5-D) post-transection in the section adjacent to the one shown in “F”. Shh localization is largely in a complementary zone of outgrowth ventral to Msi-1 expression. (Combined Msi-1 and Shh expression shown in Supplementary Figure 6.) The dorsal portion of the notochord is visible below the outgrowth (noto). (H) Cross section of lesion site in NF 52 spinal cord 10-days (10-D) post-transection showed the reconstructed central canal with ependymal Msi-1 expression. (I) NF 52 spinal cord 10-days post-transection (10-D) in the section adjacent to the one in “H” showed Shh expression restored to floorplate location. Differential interference contrast optics, transmitted light images. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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