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FIGURE 8. Axolotl mRNA (purple) or Msi antibody (red) tissue localization and DAPI nuclear label (blue). (A) Late tailbud embryo (BD 42–44) cross-section in situ hybridization for Msi-1 mRNA showed localization in ependymal region around the central canal and in gray matter. Differential interference contrast image. (B) Antibody localization in late tailbud embryo sections showed Msi-1 protein was present in the same regions as the mRNA shown in “A.” (C) Juvenile cord maintained Msi-1 expression in the ependymal zone, extending dorsally. (D) Intact adult Axolotl cord showed no Msi-1 protein expression, only DAPI-stained nuclei (blue). (E) Parasagittal section of 2 weeks adult regenerative outgrowth showed strong upregulation of ependymal Msi-1expression. (F) In cross section, at higher magnification than in “E” the mesenchymal nature of the Msi-1-positive 2-week adult regenerated outgrowth is shown. Fluorescence microscope images. All images are dorsal side up. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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