XB-IMG-171807
Xenbase Image ID: 171807
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FIGURE 11. Xenopus and Axolotl cell proliferation; PCNA antibody (red) and DAPI nuclear stain (blue) are used throughout. (A) PCNA staining in NF 50 spinal cord. The ependymal layer cells are PCNA-positive. The PCNA labeled region coincides with the region of Msi-1-positive cells shown in Figure 7A. (B) NF 62 spinal cord with PCNA antibody and DAPI staining. Some cells in the ependymal layer are still proliferating, but in a reduced area around the central canal. (C) Parasagittal section shows regenerative outgrowth 7-days (7D) post-transection regenerating NF 50 tadpole spinal cord. The zone of outgrowth and lesion site is indicated within the dashed line. The entire ependymal population contains proliferating cells. (D) Intact juvenile Axolotl (∼12 cm; less than 6 months old) spinal cord cross section shows extensive ependymal and gray matter PCNA labeling. There is a substantial dorsal extension of labeled ependymal cells. (E) Intact adult Axolotl (∼25 cm; approximately 2–3 years old) spinal cord cross section shows strong, but reduced PCNA labeling in the ependymal zone. The dorsal plume of PCNA-labeled cells no longer exists and labeling is reduced in the gray matter laterally. (F) Parasagittal section showing PCNA-positive cells in mesenchymal ependymal outgrowth. (Supplementary Figure 8 shows DIC image of the section with the outgrowth and stump region labeling for orientation.) Fluorescence microscope images. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |