XB-IMG-171808
Xenbase Image ID: 171808
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FIGURE 12. Xenopus, Axolotl cell culture in EGF-containing culture medium (+EGF). (A–C) Phase contrast images. (A) Control NF 50 Xenopus ependymal cells in +EGF, 1 day (1D) 6 to 8 large patches of cells adhere to each dish. (B) NF 64 cultures: small, islands of cells, 4–6/dish, exhibit poor attachment and growth even at 8 days (8D) in culture. (C) Control adult Axolotl 2 weeks regenerating ependymal outgrowth explants in EGF at 3 days (3D) culture, patches of cells continue to expand in culture. (D,E) Fluorescent-Phalloidin probes are used to show the organization of F-actin in Xenopus and Axolotl +EGF cultures. (D) Alexa fluor 488-Phalloidin green fluorescence in a +EGF Xenopus ependymal culture shows typical mesenchymal cell F-actin organization, parallel to the long axis. (E) Rhodamine-Phalloidin red fluorescence in a +EGF Axolotl culture shows cell F-actin organization typical of mesenchymal outgrowth. DAPI nuclear stain used in “D,E” (blue). (D,E) Fluorescence microscope images. Image published in: Chernoff EAG et al. (2018) Copyright © 2018 Chernoff, Sato, Salfity, Sarria and Belecky-Adams. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |