Xenbase Image ID: 171811

Figure 1. Y RNA-Independent Initiation Activity in Xenopus Egg Extracts(A) Y RNA-dependent initiation of DNA replication in a human cell-free system. Human late G1 phase template nuclei were incubated in either buffer (top) or mock-treated or Y RNA-depleted cytosolic extracts.(B) Y RNA-independent initiation of DNA replication in Xenopus egg extracts. Human late G1 phase template nuclei were incubated in either buffer (top) or mock-treated or Y RNA-depleted Xenopus egg extracts. Representative immunofluorescence micrographs are shown with merged channels for total DNA (propidium iodide, red) or sites of DNA replication (digoxigenin-deoxyuridine triphosphate [dUTP] incorporation, green).(C) Experimental design for the detection and isolation of the initiation activity from Xenopus egg extracts by cross-species complementation assays.(D) Cross-species complementation assays. Left: template nuclei were incubated as in (A) in either buffer or mock-treated cytosolic or Y RNA-depleted human cytosolic extracts. Right: increasing amounts of Y RNA-depleted Xenopus egg extracts were added to replication initiation reactions in the presence of either buffer (light green) or Y RNA-depleted human cytosolic extract (dark green). Percentages of replicating template nuclei for each reaction are shown as mean values ± SD of n independent experiments.Scale bars, 10 μm. Image published in: Christov CP et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page