Xenbase Image ID: 171812

Figure 2. Isolation and Identification of the Y RNA-Independent Initiation Factor from Xenopus Egg Extracts(A) Schematic representation of the fractionation scheme.(B) Activity profile of the sucrose minigradient fractions. Template nuclei were incubated in Y RNA-depleted human cytosolic extract supplemented with the indicated gradient fractions. Percentages of replicating nuclei are shown as mean values ± SD of n = 4 independent fractionation experiments. Brackets indicate results of t tests (unpaired, two-tailed with unequal variance) of experimental samples (fractions 6 and 7) against the control (∗∗∗p ≤ 0.001). Tests of all other fractions against the control were not significant. Sedimentation of calibrator protein complexes (cytochrome c, 12.5 kDa; BSA, 68 kDa; aldolase, 158 kDa; catalase, 240 kDa) are indicated at the top.(C) Proteins in active fractions as identified by mass spectrometry. Proteins identified in both of two independent experimental replicates that were enriched in the active gradient fractions 6/7 compared with inactive fraction 5 are ranked according to their abundance (determined as emPAI scores).(D) NuRD subunit sedimentation profiles. Fractions of the sucrose minigradient were analyzed by western blotting with antibodies specific for the indicated NuRD subunits.See also Figure S1 and Table S1. Image published in: Christov CP et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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