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Figure 5. Inhibition of xNuRD Leads to an Inhibition of Y RNA-Independent DNA ReplicationShown is inhibition of DNA replication using HDAC inhibitors and NuRD-specific antibodies.(A) Template nuclei were incubated in Y RNA-depleted human cytosolic extract supplemented with partially purified xNuRD and the indicated concentrations of the HDAC inhibitors trichostatin A (TSA), SAHA, and MS-275.(B) Template nuclei were incubated with human cytosolic extract containing human NuRD and Y RNAs in the presence of the indicated inhibitors.(C) Template nuclei were incubated in Y RNA-depleted human cytosolic extract supplemented with partially purified xNuRD and the indicated NuRD-specific antibodies.(D) Template nuclei were incubated with human cytosolic extract containing human NuRD and Y RNAs in the presence of the indicated NuRD-specific antibodies. Mean values ± SD of the percentages of replicating template nuclei are plotted from n independent experiments. Results of t tests (unpaired, two-tailed with unequal variance) of treated experimental samples against the no addition controls are indicated (∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001). Image published in: Christov CP et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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