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Fig 6. Dsel is expressed maternally and in the eye, brain and cranial neural crest. Embryos after whole-mount in situ hybridization are shown in animal view (A), lateral view (B,E,E’,I,I’), hemi-sectioned (C) and transversally sectioned (F-H). (A) 4-cell stage embryo. (B,C) Blastula embryos. The bold line indicates the level of section in C. (D) qPCR analysis at stage 9. Note ubiquitous expression of Dsel in the animal cap, marginal zone and vegetal region. (E-I’) Tailbud embryos. The bold lines indicate the level of sections in F-H. Note Dsel expression in the eye, interneurons of the mid- and hindbrain (arrows), cranial neural crest (indented arrowheads) and bilateral trigeminal ganglia. an, animal cap; ey, eye; hb, hindbrain; ma, marginal zone; mb, midbrain; V, trigeminal ganglion, vg, vegetal region. Scale bars are 500 μm. Image published in: Gouignard N et al. (2018) © 2018 Gouignard et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue dsel.L C18orf4, LOC108717926 X. laevis Throughout NF stage 3 (4-cell) animal hemisphere dsel.L C18orf4, LOC108717926 X. laevis Throughout NF stage 9 dorsal animal cap animal cap inner layer animal cap outer layer blastocoel floor blastocoel roof dsel.L C18orf4, LOC108717926 X. laevis Throughout NF stage 27 optic vesicle midbrain hindbrain interneuron trigeminal ganglion dsel.L C18orf4, LOC108717926 X. laevis Throughout NF stage 29 and 30 eye brain spinal cord trigeminal nerve cranial neural crest Image source: Published Larger Image Printer Friendly View

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