XB-IMG-171840
Xenbase Image ID: 171840
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Supplementary Figure 1: (A) The antisense Xic1Mo morpholino oligonucleotide Xic1Mo (red) binds to the first 25-nucleotide sequences of p27Xic1 RNA (black) while the Control Morpholino ConMo (blue) with 5-nucleotide substitutions cannot, as detected by Western blotting for Xic1 protein (B). The Xic1 mutant RNA (green) used for rescue experiments was designed to contain 8 transcriptionally silent nucleotide substitutions at the 5’ end. (B) Xic1Mo, but not CTRMo, inhibits the in- vitro translation of wild-type Xic1 but cannot target the nucleotide-substituted Xic1 rescue construct. (C) Western blot demonstrating that injection of Xic1Mo effectively inhibits the expression of Xic1 protein in stage 17 embryos. (D) A ventral and lateral view of stage 33/4 Xic1Mo injected embryo following TIc ISH and clearing with Benzyl Benzoate: Benzyl alcohol, compared to staged matched uninjected embryo.
Image published in: Movassagh M and Philpott A (2008) Published on behalf of the European Society of Cardiology. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license
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