XB-IMG-171895
Xenbase Image ID: 171895
Fig. 7. Effect of chemicals on es1:EGFP and endogenous es1 expression. (A, B) Successive fluorescence images of tail–amputated line 1 tadpoles which were treated with DMSO (Control) or TGF-β signal inhibitor (TGFβ i, SB431542). Time points after tail amputation are indicated. (C-F) Representative EGFP fluorescence images at 24 hpa in tadpoles treated with DMSO (Control), or inhibitor for ERK (ERK i, U0126), ROS (ROS i, DPI) or JNK (JNK i, SP600126) signal. A pair of arrows indicates the amputation plane. Arrowheads indicate the posterior margins of the amputated stump. Anterior left, dorsal up. Bars, 500 µm. (G-I) Expression level of es1:EGFP at 12 hpa in tail stump was quantified with ImageJ software. Relative mean values are shown with standard errors. ***P<0.001; Student's t-test. (J, K) Relative expression level of es1 compared to that in wild-type tadpoles at 12 hpa was determined by qRT-PCR after tail amputation. Tail-amputated tadpoles were treated with the indicated chemicals. Mean value of four (J) or six (K) biological replicates is shown with standard error. *p<0.05; Student's t-test.
Image published in: Sato K et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |