Xenbase Image ID: 171929

Supplemental Figure S1. Depletion of MarvelD3 and neural crest development. (A) gfp-fl marveld3 was inserted in pCS2+ vector (BamH1/EcoR1). 7mut-marveld3 was subcloned in pCS2+ vector (BamH1/ EcoR1). Restriction sites: underlined; mutated bases of marveld3: underlined lower cases. (B) neural crest induction (stage 15) was determined by WISH for twist, snai1, foxd3 and snai2/slug in control morpholino- (i), MD3A morpholino- (ii), MD3B morpholino-injected embryos (iv) and embryos co-injected with FL marveld3 (iii) or 7mut-marveld3 mRNA (v), respectively; the number of embryos analysed is indicated at the bottom right of each panel; red asterisk, injected side of the embryo with light blue β- galactosidase staining; purple corresponds to the WISH staining; scale bar, 500 μm. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 15 neural crest neuroectoderm snai1.L LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 15 neuroectoderm neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 15 neuroectoderm neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 15 neuroectoderm neural crest Image source: Published Larger Image Printer Friendly View

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