XB-IMG-171930
Xenbase Image ID: 171930
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Supplemental Figure S2. Effect of MarvelD3 depletion on neural plate border specification. (A, B) Neural plate border specification was established by WISH against sox2, epk/xk81a1, pax3 and six1 in control morpholino- (A, i; B, i), MD3A morpholino- (A, ii; B, ii), MD3B morpholino-injected embryos (A, iv; B, iv) and embryos co-injected with fl marveld3 (A, iii; B, iii) or 7mut-marveld3 mRNA (A, v; B, v) at stage 15. Scale bars, 500 μm; (A) red and green bars demarcate the medio-lateral expansion of sox2; (A, B) black dotted line identify the midline; (B) white bracket indicate the distance from the midline; the number of embryos analysed is indicated at the bottom right of each panel; red asterisk, injected side of the embryo with light blue β-galactosidase staining. Scale bars, 500 μm; red and green bars demarcate the medio-lateral expansion of sox2; black dotted line identify the midline; the number of embryos analysed is indicated at the bottom right of each panel; red asterisk, injected side of the embryo with light blue β- galactosidase staining; purple staining corresponds to WISH. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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