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Supplemental Figure S3. MarvelD3 depletion and mesoderm formation. Effect of MD3AB morpholinos on the mesoderm was analysed by WISH against xbrachyury (xbra; A, B) and goosecoid (gsc; C, D) at stage 11; ventral view. Scale bar, 500 μm; the number of embryos analysed is indicated at the bottom left of each panel; light blue indicates the β-galactosidase staining on the injected side of the embryo while purple corresponds to WISH staining. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tbxt.L bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 mesoderm upper blastopore lip dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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