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Fig. 3. Dnd1 binds nanos1 RNA. (A) Double-filter nucleic acid-binding assay shows that recombinant Dnd1 protein binds nanos1 RNA. The experiment was repeated twice. (B) RIP assay followed by RT-PCR shows that endogenous Dnd1 protein selectively binds a subset of germline RNAs in stage 7 embryos. The experiment was performed three times. (C) RNAs were pulled down by GST-Dnd1 and measured by qPCR. Ratio between the pull down and 5% of RNA input is shown. GFP served as a negative control. Data are shown as mean±s.d. **P<0.01. The experiment was repeated four times. (D) nanos1-nanos1 3′UTR and nanos1-β-globin 3′UTR were injected into stage VI oocytes with or without dnd1 mRNA. Nanos1 protein was immunoprecipitated and analyzed by western blot for Nanos1 and Dnd1. The experiment was repeated twice. Image published in: Aguero T et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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