Xenbase Image ID: 171936

Fig. 4. Dnd1 physically interacts with eIF3f. (A) Anti-FLAG (lanes 4-6) or anti-Myc (lanes 10-12) antibodies were used to IP eIF3f or Dnd1 from cell lysates (lanes 9,12). Addition of RNaseA to lysates did not disrupt the Dnd1-eIF3f complex (lane 12). (B) Co-IP shows the interaction between myc-Dnd1 and endogenous eIF3f in HEK293T cells. IgG served as negative control. (C) Co-IP shows that myc-Dnd1 formed complexes with FLAG-eIF3 h and FLAG-eIF3m in HEK293T cells. Experiments were repeated three times. Image published in: Aguero T et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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