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Fig. 5. Interaction between endogenous Dnd1 and eIF3f in Xenopus embryos. (A) Egg or embryo extracts were fractionated on 7-20% sucrose gradients. Gradient fractions were blotted with antibodies for Dnd1, eIF3f and eIF3c proteins. Experiments were repeated four times. (B) Fractions 1+2 and fractions 8+9+10 of embryo extracts from the sucrose gradients were pooled and immunoprecipitated with an anti-Dnd1 antibody. Endogenous eIF3f and Dnd1 proteins were monitored by western blot. Experiments were repeated three times.

Image published in: Aguero T et al. (2017)

Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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