Xenbase Image ID: 171938

Fig. 6. Interaction between Dnd1 and eIF3f is essential for nanos1 translation. (A) FLAG-tagged eIF3f deletions were transfected into HEK293T cells along with myc-Dnd1. Lysates were immunoprecipitated with anti-FLAG antibody and analyzed by western blot. The minimal Dnd1-binding domain was mapped to residues 92-200 of eIF3f, which is indicated by the red asterisk. (B) Schematic summarizing experiments shown in A. +, Dnd1 binding; −, lack of Dnd1 binding. (C) Myc-Dnd1 and FLAG-eIF3f were transfected into HEK293T cells with increasing amounts of eIF3f92-200. Cell lysates were immunoprecipitated with an anti-myc antibody and analyzed by western blot. (D) Myc-Dnd1 and FLAG-eIF3h were transfected into HEK293T cells in the presence or absence of eIF3f92-200. Cell lysates were immunoprecipitated with an anti-myc antibody and analyzed by western blot. Experiments were repeated three times. (E) nanos1 RNA was injected into fertilized eggs alone or with eIF3f92-200, or with eIF3f92-200 and myc-dnd1 RNAs. At stage 11, Nanos1 protein was immunoprecipitated and analyzed by western blot. Non-specific band served as a loading control. The experiment was performed twice. Image published in: Aguero T et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page