Xenbase Image ID: 171967

Fig. 4. Blebbistatin-induced cell spreading. (A) Stills from a timelapse series (Movie S5) in top- and side-view of individual NCCs explanted onto fibronectin. Blebbistatin (50 μM) was added 35 min after cell seeding. Bar 10 μm (upper panel) and 20 μm (lower panel). (B) Quantitating bleb area per cell as a measure of intracellular tension and cell spread area of two representative cells (colors denote same cells). Addition of blebbistatin (50 μM) after 35 min. (C) Percentage of spread cells over time in control and blebbistatin-treated cell samples. Quantification started after blebbistatin (50 μM) addition 35 min after cell seeding. (D) Time of cell spreading (mean ± SD) of control and blebbistatin-treated cell samples. Numbers in the data bars denote number of tested cells per condition. (E) Young’s moduli (mean ± SD) of rounded-up and spread control cells and blebbistatin-treated spread cells. Numbers in the data bars denote number of tested cells per condition. Significance according to the Mann-Whitney-Test, with * = p < 0.05. Image published in: Blaue C et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page