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Fig. 6. Model depicting the effect of cadherin-11 modulation and blebbistatin treatment on cortical tension and the dynamics of single NCC (left panel) and NCC cluster spreading (right panel) on fibronectin. Left panel: Single cells initially display dynamic membrane blebbing after seeding on fibronectin, which later gradually decreases followed by cell spreading. Cadherin-11 overexpression or reducing intracellular contractility by blebbistatin accelerates NCC spreading over wildtype levels. In contrast, cadherin-11 depletion prevents cell spreading and maintains sustained membrane blebbing. Treatment of cadherin-11 KD cells with blebbistatin reduces blebbing, but fails to induce cell spreading, consistent with an additional requirement for cadherin-11 in substrate adhesion to fibronectin. Right panel: Multilayered NCC cluster initially display strong blebbing, indicating high cellular tension immediately after seeding on fibronectin. After flattening into a monolayer, the cell sheet features contractile ring at the periphery. Delaminating cells display reduced stiffness, but enhanced spreading and substrate traction. Overexpression of cadherin-11 accelerates cell spreading and delamination. Blebbistatin treatment reduces peripheral stiffness to average cell sheet levels, but does not enhanced leader cell emigration. Cadherin-11 depleted clusters do not spread and expand, regardless of stiffness. Green indicates membrane areas with low tension, red with high tension. A timeline progresses from left to right. Image published in: Blaue C et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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