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Suppl. Figure S4 (A) Stills from a timelapse series depicting the spreading of cadherin-11 knockdown NCCs. Upper row: three cell clusters collected from the non-injected control sides of embryos. Lower row: three clusters collected from the morpholino-injected sides of the same three embryos. Clusters from the injected and the corresponding non-injected sides were placed side-by-side and timelapse recording was started 10 min after cluster seeding. Images were collected every 2 min for a total duration of 12 h. Blebbistatin (50 µM) was added to the sample 3.5h after explantation. The complete timelapse series is contained in Movie S9. (B) Quantification of cell cluster area over time.

Image published in: Blaue C et al. (2018)

Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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