XB-IMG-171998
Xenbase Image ID: 171998
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Figure 2. HIF-1 activity is required to induce re-initiation of proliferation in nutrient-deprived hypoxic retinas. (A) Western blot of retinas isolated from normal-fed or nutrient-deprived animals kept under normal oxygen concentrations or in hypoxia, probed for HIF-1α subunit and α-tubulin. HIF-1α is stabilized under hypoxia in both normal-fed and nutrient-deprived animals. At least 22 retinas were taken for each condition; all conditions in each experiment have the same number of retinas (n = 5). (B) Quantification of the Western blot performed in (A). Error bars represent standard deviations. Two hooked ends of horizontal lines indicate the two conditions compared, * p-value < 0.05, *** p-value < 0.001; n = 5. (C–H) EdU incorporation in DAPI-stained retinas from normal-fed animals (C–E) kept in normoxia (C), hypoxia (D) or incubated with Echinomycin under hypoxia (E) and in nutrient-deprived animals kept in normoxia (F), hypoxia (G) or incubated with Echinomycin under hypoxia (H). Nutrient-deprived retinas no longer resume their proliferation in hypoxia when function of HIF-1 is blocked by Echinomycin. Scale bars = 50 µm. In each condition, a minimum of 10 retinas was examined. (I) Quantification of the experiment performed in (C–H). Error bars represent standard deviations between three independent experiments. Two hooked ends of horizontal lines indicate the two conditions compared, * p-value < 0.05; n > 3. For each condition, between 10 and 15 retinas were quantified. Image published in: Khaliullina H et al. (2016) © 2016 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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