Xenbase Image ID: 172000

Figure 4. Block in glutaminolysis interferes with re-initiation of proliferation in nutrient-deprived hypoxic retinas. (A–F) EdU incorporation in DAPI-stained retinas from normal-fed animals (A–C) kept in normoxia (A), hypoxia (B) or incubated with BPTES under hypoxia (C) and in nutrient-deprived animals kept in normoxia (D), hypoxia (E) or incubated with BPTES under hypoxia (F). Nutrient-deprived retinas no longer resume their proliferation in hypoxia when glutaminolysis is blocked by BPTES. Scale bars = 50 µm. In each condition, a minimum of 8 retinas was observed. (E) Quantification of the experiment performed in (A–F). Error bars represent standard deviations between three independent experiments. Two hooked ends of horizontal lines indicate the two conditions compared, *** p-value < 0.001, n = 3. For each condition, between 8 and 17 retinas were quantified. Image published in: Khaliullina H et al. (2016) © 2016 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + hypoxia + NF38 Anatomical Phenotype decreased cell population proliferation in retinal progenitor cell region Xla Wt + nutrient deprivation + hypoxia + NF38 Anatomical Phenotype decreased cell population proliferation in retinal progenitor cell region Xla Wt + nutrient deprivation + hypoxia + BPTES + NF 41 + 24h Anatomical Phenotype decreased cell population proliferation in retinal progenitor cell region Larger Image Printer Friendly View

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