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Figure S4. Complex formation of xNuRD in Xenopus egg extracts. Related to Figure 6. xNuRD was partially purified by precipitation of egg extract with 20-45% ammonium sulphate and ultracentrifugation through preparative sucrose gradients. MTA2, HDACm, HDAC1 and RBBP7/p46 subunits were analysed by Western blot analysis of the preparative sucrose gradient fractions. Fraction numbers (1-10, collected from top to bottom of the gradient), the pellet fraction (P), and positions of sedimentation markers are indicated. Image published in: Christov CP et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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