Xenbase Image ID: 172082

Figure 4. Loss of PAWS1 inhibits Wnt signalling at the level of the destruction complex A. Relative TOPFlash luciferase activity of PAWS1WT and PAWS1−/− U2OS cells after treatment with conditioned media (L‐CM) or Wnt3A‐conditioned media (L3‐CM) (n = 3; error bars represent ± SEM). B. Wnt3A‐induced activation of the target genes AXIN2 and CYCLIN D1 was examined by qPCR at the indicated time points in PAWS1WT and PAWS1−/− U2OS cells. Transcript expression data are represented as fold induction over unstimulated control and are internally normalised to β‐actin control. Error bars represent ± SEM (***P < 0.0001; two‐way ANOVA with multiple comparisons; n = 3). C, D Epistasis analysis of xPAWS1 in the canonical Wnt pathway. Xenopus embryos were injected at the one‐cell stage with the indicated mRNAs, and then at stage 10, animal caps were assessed by qPCR for the expression of Siamois. xPAWS1 acts downstream of the Wnt receptor LRP6 (C) at the level of the destruction complex (D) (n = 3 error bars represent ± SD). E. Relative TOPFlash luciferase activity of PAWS1WT and PAWS1−/− U2OS cells after treatment with either conditioned medium (L‐CM), Wnt3A‐conditioned medium (L3‐CM) or 5 μM of the GSK3 inhibitor CHIR99021 for 6 h. Data are normalised to Renilla‐luciferase internal control (n = 4; error bars represent ± SEM). Image published in: Bozatzi P et al. (2018) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page