Xenbase Image ID: 172086

Figure 7. PAWS1 association with CK1α is essential for induction of Wnt signalling PAWS1F296A, PAWS1F296A/F300A and PAWS1D262A mutants that do not interact with CK1α fail to induce a secondary axis in Xenopus embryos. Relative TOPFlash luciferase activity of U2OS WT, PAWS1−/−, PAWS1WT and PAWS1F296A rescue cells after treatment with control‐conditioned media (L‐CM), Wnt3A‐conditioned media (L3‐CM) (n = 3; error bars represent ± SEM). Human CK1α induces a secondary axis in Xenopus embryos in a dose‐dependent manner. Co‐expression of PAWS1 and CK1α mRNAs do not induce secondary axis. Effect of CK1 isoforms on PAWS1‐dependent axis‐inducing activity. 250 pg of xPAWS1 and 300 pg of the indicated CK1 isoform mRNAs were injected into one ventral blastomere at the four‐cell stage. Axis induction was assessed at stage 28. PAWS1 interacts with kinase‐dead CK1α in U2OS cells. U2OS cells were co‐transfected with HA‐CK1α (WT or KD) and FLAG‐PAWS1, and cell extracts were subjected to FLAG IP, followed by SDS–PAGE and Western blot analysis with the indicated antibodies. Image published in: Bozatzi P et al. (2018) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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