Xenbase Image ID: 172087

Figure EV4. xPAWS1 does not induce nuclear localisation of NFAT Dissociated animal cap cells injected with either 500 pg of NFAT_GFP or 500 pg of NFAT_GFP and 250 pg of xPAWS1_mCherrymRNA. Images are maximum intensity projections, scale bars = 50 μm. Nuclear localisation of NFAT_GFP was assessed before and after treatment with 0.025 μg/ml calcium ionophore. ns: no statistical significance (n = 4; error bars represent ± SD; Mann–Whitney test, unpaired, two‐tailed with unequal variance, P = 0.6857). Expression of a catalytically inactive CK1α leads to Ca2+‐independent nuclear localisation of NFAT_GFP. Dissociated animal cap cells injected with either 500 pg of NFAT_GFP alone and 250 pg of H2B_RFP (top panels), 500 pg of NFAT_GFP, 250 pg H2B_RFP and 250 pg of MTxPAWS1 (middle panels), or 500 pg NFAT_GFP, 250 pg H2B_RFP, and 300 pg CK1α KD (bottom panels). Images represent a single z‐frame at the level of the nucleus. Cells were imaged using a Zeiss LSM710 confocal microscope controlled with Zen Black software. Image analysis was performed using Zen blue software. Scale bars = 50 μm. Image published in: Bozatzi P et al. (2018) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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