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Figure 8. PAWS1 does not appear to affect Axin1 and β‐catenin complexes upon Wnt signalling U2OS wild‐type (WT) and PAWS1−/− (KO) cells were treated with control‐conditioned medium or Wnt3A‐conditioned medium, and the extracts (0.5 mg protein) were subjected to immunoprecipitation using antibodies against the endogenous CK1α, AXIN1 and β‐catenin antibodies or anti‐rabbit pre‐immune IgG as a control (10 μg antibodies coupled to 10 μl packed protein‐G sepharose beads). IPs were resolved by SDS–PAGE and immunoblotted with the indicated antibodies.Source data are available online for this figure. Image published in: Bozatzi P et al. (2018) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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