XB-IMG-172140
Xenbase Image ID: 172140
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FIG. 4. Developmental expression of xGATA-binding protein
and larval globin genes. (A) Total cellular RNAs (1 /g) from staged
Xenopus embryos were subjected to RT-PCR using specific primers
for ,T1 globin, xGATA-1, xGATA-2, xGATA-3, and xMyoD. Controls
consisted of RT-PCR products from total RNA from Xenopus
adult erythroid cells and HeLa cells and H20. Lanes: 1, oocyte; 2,
4-cell embryo; 3, 32-cell embryo; 13, erythroid cells; 14, HeLa cells;
15, H20. The Nieuwkoop stage is indicated in the following lanes.
Lanes: 4, 7; 5, 9; 6, 11; 7, 12.5; 8, 13; 9, 18; 10, 24; 11, 26; 12, 30. (B)
Total cellular RNAs from staged Xenopus embryos were subjected
to Northern blot analysis and hybridized with an aT1 globin cDNA
probe (37). Nieuwkoop stages are as follows. Lanes: 1, 10.5; 2, 12;
3, 13; 4, 15; 5, 17; 6, 20; 7, 24; 8, 26; 9, 30; 10, 33; 11, 38; 12,
erythroid-cell control. Image published in: Zon LI et al. (1991) Copyright © 1991. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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