Xenbase Image ID: 172206

Figure 2. Biochemical and functional analyses of the currents induced by the expression of 8E-mCherry in Xenopus oocytes. (A) Time course between 2 and 4 days after oocyte injection of the mean values of currents at 60 mV for uninjected oocytes (n ≥ 7), oocytes injected with 8E-mCherry (n ≥ 18), and oocytes injected with 8A-VFP/8E-mCherry (n ≥ 7). Data are from at least two different injections and indicate the mean ± SEM; (B) Western blots against some LRRC8 proteins, using β-tubulin as a loading control. We used protein extracts of (from left to right): collagenase-treated uninjected oocytes “C Un-inj” and oocytes injected with 8A or 8D “hum 8A/8D inj”. Three independent Western-blot experiments to detect Xenopus LRRC8A and two WB experiments to detect Xenopus LRRC8D gave similar results; (C) Comparison of the mean values of current at 60 mV between uninjected oocytes (n = 4) and oocytes co-expressing 8E-mCherry (20 ng of complimentary ribonucleic acid (cRNA)) plus 10 ng of an oligonucleotide sense “x8A sense” (n = 5 at day 3, n = 5 at day 4) or plus 10 ng of an oligo antisense “x8A Asense” (n = 4 at day 3, n = 5 at day 4). Data indicate the mean ± SEM * p < 0.05, ** p < 0.01, *** p < 0.001. Another injection gave similar results. Image published in: Gaitán-Peñas H et al. (2018) © 2018 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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