Xenbase Image ID: 172207

Figure 3. Surface analysis of LRRC8 proteins expressed in Xenopus oocytes. Surface expression was examined using a chemiluminescence technique detecting the HA epitope inserted into the first extracellular loop (HAloop) already described [51]. The sequence of LRRC8A with the HA tag was CLPCKWYPYDVPDYAVTKDSC and the sequence of LRRC8E with the HA tag was CLPNHEYPYDVPDYALQENLS (HA tag in italics). The HA tag did not interfere with the proper function of the proteins. In the panel on the left, we show the membrane levels of 8A-VFP, and in the other panel, the levels of 8E-mCherry. We show here a typical experiment with n = 15, 15, and 10 oocytes for the panel on the left, and n = 8, 15, and 9 for the panel on the right. Image published in: Gaitán-Peñas H et al. (2018) © 2018 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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